Fluid Biomarkers with Deep Phenotyping in Patients with ALS (ALSA-BIO3)
The goal of the study is to continue to build the Northeast ALS Consortium (NEALS) biorepository with this collection protocol to associate biofluid collection with specific measures of upper and lower motor neuron function.
Disease:Amyotrophic Lateral Sclerosis (ALS), Sporadic ALS
Study Type:Observational Study
Study Category:biological samples
Study Chair(s)/Principal Investigator(s):
Shafeeq Ladha, Barrow Neurological Institute
Clinicaltrials.gov ID (11 digit #):NCT02819765
Coordinating Center Contact InformationBarrow Neurological Institute
Phoenix, Arizona 85013 United States
Full Study Summary:
The Northeast ALS Consortium (NEALS) biorepository is an existing resource which has provided scientists with a wide range of samples associated with clinical information. Our goal is to continue to build this repository with this collection protocol to associate biofluid collection with specific measures of upper and lower motor neuron function. This project will compliment others to upgrade the NEALS biorepository infrastructure, including updating the computer systems, expanding the number of sample freezers and establishing a repository in the Western United States, and strengthening the standard operating procedures for the repository. The project also supports existing efforts to expand the biorepository by collecting new blood and spinal fluid samples from people with ALS. This is a multicenter, non-interventional, longitudinal study in patients with ALS. There will be four (4) subject visits in this study: Baseline, month 6, month 12, and month 18. At each visit, subjects will have biofluids collected, and be evaluated with assessment tools that focus on upper and lower motor neuron burden as well as cognitive function.
The primary objective of the study is to obtain information on patients studied at regular intervals over 18 months, in conjunction with biofluid collection over that same time period. The secondary objective of the study is to integrate data from this study to other ongoing projects via the NEALS biorepository, and future collections now being planned. The outcome measures of this study are as follows:
• Motor unit number estimation will be performed on bilateral upper extremity muscles using the multipoint incremental technique (MIMNUE)
• Vital capacity, measured using slow vital capacity (SVC)
• Lower motor neuron excitability will be assessed with threshold tracking nerve conduction studies (ttNCS) in the least affected intrinsic hand muscle
• Cognitive abnormalities will be assessed using the ALS Cognitive Behavior Screen (ALS-CBS™)
• Upper motor neuron burden will be assessed using transcranial magnetic stimulation (TMS), employing a paired pulse protocol and recording from the least affected upper extremity intrinsic hand muscle
• Hand held dynamometry (HHD) will be performed on 9 muscle groups tested bilaterally
• Global function (ALSFRS-R)
At each visit, blood will be collected and banked for biomarker discovery; CSF will be collected at baseline, month 6, and at month 18.
Study Sponsor:The ALS Association and Fulton Family Foundation
4 visits over 18 months
Estimated Study Start Date:02/07/2017
Estimated Study Completion Date:12/31/2020
Posting Last Modified Date:07/29/2020
Date Study Added to alsconsortium.org:06/06/2016
In this informational webinar, Dr. Shafeeq Ladha reviews the BIO3 study design and answers questions about the study: https://www.neals.org/for-people-with-als-caregivers/educational-webinars/alsa-bio3-building-future-success-through-biomarkers
Time since Symptom Onset:
Time since Diagnosis:<24 months
Can participants use Riluzole?Yes
- Participants with sporadic ALS diagnosed as possible, laboratory-supported probable, probable or definite according to the World Federation of Neurology El Escorial criteria.
- Expected to survive >1 year (12 months) after enrollment.
- Male or female, aged 18-75.
- Ability to medically undergo lumbar puncture (LP) as determined by the investigator (i.e., no bleeding disorder, allergy to local anesthetics, prior lumbar surgery which might make LP difficult, a skin infection at or near the LP site, or evidence of high intracranial pressure).
- Willingness and medical ability to comply with scheduled visits, LP for CSF collection, laboratory tests, and other study procedures.
- Ability to understand the purpose and risks of the study and provide signed and dated informed consent and authorization to use protected health information (PHI) in accordance with national and local subject privacy regulations.
- Geographic accessibility to the study site.
- Any contraindications to having an LP, including but not limited to: Platelet count <100,000/µL.
- History of bleeding disorder.
- History of intolerance to the LP procedure.
- Evidence of topical or other skin infection at the LP site.
- History of allergy or other adverse reaction to local anesthetics used in the study.
- History of traumatic central nervous system injury or stroke.
- Other unspecified reasons that, in the opinion of the Investigator, make the subject unsuitable for enrollment.
Additional criteria for sites performing TMS:
- Inability to perform either TMS or NCS studies due to insufficient motor evoked potential (MEP) or compound muscle action potential (CMAP) amplitude.
- Contraindication to TMS studies including ferromagnetic metal in the head or neck (potentially found in aneurysm clips, implanted medication pumps, implanted brain stimulators, pacemakers, cochlear implants), or history of epilepsy. Dental fillings are permitted.
Additional criteria for sites performing MRI cytography:
- Subjects who have a history of claustrophobia that cannot be adequately controlled.
- Subjects who have a physical limitation related to fitting in the bore of the magnet.
- Subjects who have a history of allergic reaction to contrast agents.
- Subjects with a pacemaker, epicardia pacemaker wires, MRI-incompatible cardiac valve prostheses, MRI-incompatible vascular clips.
- Subjects with MRI-incompatible cochlear implants.
- Subjects with spinal nerve simulators.
- Subjects with an infusion pump.
- Subjects with metallic fragments in the eyes/orbits or in the vicinity of the brain or major neurovascular structures of the body, subjects with an employment history which involves exposure to welding, or subjects who have shrapnel any place in their body.
- Subjects with acute kidney injury or renal insufficiency (eGFR of <20 ml/min/1.73 m^2) as they are at increased risk of Nephrogenic Systemic Fibrosis following administration of gadolinium-based MRI contrast agents.
- Subjects unable to lay supine in the magnet because of orthopnea.
Site Contact Information
Barrow Neurological Institute
Phoenix, Arizona 85013
University of California, Irvine
Dept. of Neurology
Orange, California 92868
University of Michigan
Ann Arbor, Michigan 48109
Grand Rapids, Michigan 49503
Hospital for Special Surgery (HSS)
Mona Shahbazi / 212-774-2361
New York, New York 10021
Oregon Health & Science University
Portland, Oregon 97239
Philadelphia, Pennsylvania 19122
University of Pittsburgh Medical Center
Pittsburgh, Pennsylvania 15213
Medical University of South Carolina
Department of Neurosurgery & Neurology
Charleston, South Carolina 29425